Fig. 4. Expression level of ACSL1 is increased TNF-α mediated pro-inflammatory monocytes. (A) Cells were incubated with TNF-α and vehicle for 24 hours. Cells were harvested and ACSL1 mRNA was determined by real time RT-PCR. (B) ACSL-1 deficient monocytic cells were treated with TNF-α and vehicle for 24 hours. ACSL1 expression was determined by real time RT-PCR. The data in all Fig.s are representative of three independent experiments.